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bugbuster detergent solution  (Millipore)


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    Structured Review

    Millipore bugbuster detergent solution
    Bugbuster Detergent Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bugbuster+detergent+solution/bugbuster+detergent+solution/pm39862188__ja4c13307_si_001-25-32-35
    Average 90 stars, based on 1 article reviews
    bugbuster detergent solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Protein Purification:

    Article Title: Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH = 8.0) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: Electronic Structure of Two Catalytic States of the [FeFe] Hydrogenase H-Cluster As Probed by Pulse Electron Paramagnetic Resonance Spectroscopy
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer A, 50 mM HEPES, 150 mM KCl, pH = 8.0, with 1 mM freshly made sodium dithionite added) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: Electronic structure of two catalytic states of the [FeFe] hydrogenase H-cluster as probed by pulse electron paramagnetic resonance spectroscopy
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer A, 50 mM HEPES, 150 mM KCl, pH = 8.0, with 1 mM freshly made sodium dithionite added) containing 1xBugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore) and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: A [4Fe-4S]-Fe(CO)(CN)-L-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH=8.0, with 1 mM freshly-made sodium dithionite added) containing 1x Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonase (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore) and EDTA-free protease inhibitor tablet (Roche). ..

    Article Title: Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H‑Cluster
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH 8.0) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: EPR Characterization of the BlsE Substrate Radical Offers Insight into the Determinants of Reaction Outcome that Distinguish Radical SAM Dioldehydratases from Dehydrogenases
    Article Snippet: .. Protein purification was also carried out in the Coy anaerobic chamber as previously reported.1,2 Cells were lysed in HEPES buffer (50 mM HEPES, 150 mM KCl, pH 8.0) containing the following: 1× Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonase (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Protease Inhibitor:

    Article Title: Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH = 8.0) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: Electronic Structure of Two Catalytic States of the [FeFe] Hydrogenase H-Cluster As Probed by Pulse Electron Paramagnetic Resonance Spectroscopy
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer A, 50 mM HEPES, 150 mM KCl, pH = 8.0, with 1 mM freshly made sodium dithionite added) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: Electronic structure of two catalytic states of the [FeFe] hydrogenase H-cluster as probed by pulse electron paramagnetic resonance spectroscopy
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer A, 50 mM HEPES, 150 mM KCl, pH = 8.0, with 1 mM freshly made sodium dithionite added) containing 1xBugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore) and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: A [4Fe-4S]-Fe(CO)(CN)-L-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH=8.0, with 1 mM freshly-made sodium dithionite added) containing 1x Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonase (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore) and EDTA-free protease inhibitor tablet (Roche). ..

    Article Title: Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H‑Cluster
    Article Snippet: .. For protein purification, cells were lysed in a HEPES buffer (buffer W, 50 mM HEPES, 150 mM KCl, pH 8.0) containing 1 × Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonanse (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Article Title: EPR Characterization of the BlsE Substrate Radical Offers Insight into the Determinants of Reaction Outcome that Distinguish Radical SAM Dioldehydratases from Dehydrogenases
    Article Snippet: .. Protein purification was also carried out in the Coy anaerobic chamber as previously reported.1,2 Cells were lysed in HEPES buffer (50 mM HEPES, 150 mM KCl, pH 8.0) containing the following: 1× Bugbuster detergent solution (EMD Millipore), 25 U/mL benzonase (EMD Millipore), 1 kU/mL rLysozyme (EMD Millipore), and one EDTA-free protease inhibitor cocktail tablet (Roche). ..

    Lysis:

    Article Title: Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases
    Article Snippet: .. To disrupt the bacterial cell wall, lysozyme (250 mg) and BugBuster detergent solution (4.0 ml of 10×; EMD Millipore) were added to the thawed cell pellet, and cell lysis proceeded with vigorous rotation on an orbital shaker for 2 h at 4 °C. ..

    Article Title: Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases
    Article Snippet: .. To disrupt the bacterial cell wall, lysozyme (250 mg) and BugBuster detergent solution (4.0 mL of 10X; EMD Millipore) were added to the thawed cell pellet and cell lysis proceeded with vigorous rotation on an orbital shaker for 2 h at 4 °C. ..



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